* However, the specimens were dried before microtensile testing so Young's modulus values may have been greater than those of trabeculae in vivo. Ott, Susan M. “Cortical or Trabecular Bone: What’s the Difference?” American Journal of Nephrology, vol. Then, real‐time PCR with the SYBR green detection method was used to examine the expression levels of Wnt3a, Wnt 4, Wnt 5a, Wnt 7, Wnt 10a, Wnt 16, LDL receptor related protein 5 (LRP 5), LRP 6, LRP 4, β‐catenin,T cell factor (TCF), lymphoid enhancer‐binding factor 1(LEF‐1), WNT1 inducible signaling pathway protein 1 (WISP‐1), axin‐2, cyclin D1, c‐Myc, alkaline phosphatase (ALP), osteocalcin (OCN), nuclear factor‐kappaB ligand (RANKL), and osteoprotegerin (OPG).
Values are expressed as the means ± SD, *TRAP staining.
With either testing technique the mean trabecular Young's modulus was found to be significantly less than that of cortical bone (p < 0.0001).
(J) Quantitative analysis of the number of osteoblasts and osteoclasts in CA‐β‐catenin mice. (E) Quantitative analysis of the number of osteoblasts and osteoclasts in wild‐type mice. 2020 Mar 7;11:2041731420909208. doi: 10.1177/2041731420909208. © 2016 Orthopaedic Research Society. Trabecular bone fills the inner region of the long bones while cortical bone makes the outer layer of the long bones. The trabecular tissue properties were then compared to measured elastic modulus and tensile yield strain of human femoral diaphyseal cortical bone specimens obtained from a similar cohort of 34 donors.
(C) Quantitative analysis of western blotting examination in β‐catenin‐KO mice and wild‐type mice. An ultrasonic technique and microtensile testing were used to determine the Young's modulus of individual trabeculae and micro-specimens of cortical bone cut to similar size as individual trabeculae.
The extent of change is more striking in trabecular bone than in cortical bone after stabilization of β‐catenin (Fig. Specifically for bone, all factors influencing the composition, structure, and metabolic differences between cortical and trabecular bone do so through the final common cellular and signaling pathways, such as bone morphogenetic protein and Wnt mediated signaling pathway.Accumulating evidence showed that Wnt/β‐catenin signaling pathway plays a central role in bone development, bone growth and bone remodeling.The Ethical Committee of the Daping Hospital, Third Military Medical University, P. R. China, reviewed and approved the experimental protocol.
They are cylindrical in shape.
For example, β‐catenin inactivation in mesenchymal progenitors, osteoblasts and osteocytes results in low bone mass, whereas β‐catenin stabilization in mesenchymal progenitors and osteoblasts increases bone mass.Recently, it was found that manipulation of Wnt/β‐catenin signaling pathway might have different effects on trabecular bone and cortical bone. Expression of molecules related to Wnt/β‐catenin signaling and bone remodeling in wild‐type mice. Zebaze et al.
Trabecular bone and cortical bone have different bone remodeling levels, and the underlying mechanisms are not fully understood.
Taken together, these data indicate that metaphyses react more strikingly in CA‐β‐catenin mice and that the bone remodeling level increased more significantly in trabecular bone than in cortical bone.After β‐catenin was knocked‐out in mice, the mRNA expression levels of β‐catenin, TCF, LEF‐1, wisp, axin, cyclin D1, c‐Myc, ALP, and OCN were significantly down‐regulated in trabecular bone compared with wild‐type mice, and the degree of down‐regulation of the same genes in cortical bones was not as significant as in trabecular bone (Fig. Clipboard, Search History, and several other advanced features are temporarily unavailable.
The average trabecular Young's modulus measured ultrasonically and mechanically was 14.8 GPa (S.D. COVID-19 is an emerging, rapidly evolving situation.
On the other hand, trabecular bone consists of high metabolic activity. Regarding the number of osteoblasts, it decreased significantly in trabecular bone of β‐catenin knockout mice, and only decreased slightly in cortical bone of β‐catenin knockout mice compared with wild‐type mice.